Beijing Kwinbon Biotechnology Co., Ltd
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Enterobacter Sakazakii Nucleic Acid Detection Kit

—Detection Reagent, Enterobacter Sakazakii, Detection Kit, Food Safety
Product Category: Chemical Reagents
Sales Method: Export, Manufacture, Service
Payment Term: T/T, L/C, Western Union
Min. Order Unit Price
1 Box
Contact Info

Miss.Zhang

Address: China (mainland) Beijing Beijing,International Information Industry Base, Huilongguan Town, Changping District, Beijing, P. R. China

Product Detail

Product Attribute:

  • China (mainland) More >
  • KD00203
  • KWINBON
  • Detection Reagent
  • 24 Test/Kit
  • 10/16/2015
  • 10/16/2015

Product Content Description:

 1. Applications


1.1 Principle


This kit can be used inqualitative analysis of Enterobacter sakazakii contamination based on the principle of Loop-mediated isothermal amplification coupled with fluorochrome coloration reaction.

1.2 Graphy

 


2.DNA template Sample preparation 


2.1 Notice before dispose the sample

2.1.1 Pre-enrichment culture is used as the sample for detection of Enterobacter sakazakii;

2.1.2The storage of sample solution: the sample solution can be stored at 0-4 for 24 h and at -20for long term. Repeated freeze-thaw should be avoided.


2.2 The general pre-enrichment culture


2.2.1 Add 1mL of pre-enrichment culture into a sterilized 1.5mLtube and centrifuge at 10,000 r/min for 2 min. Remove the supernatant completely but not convolute the precipitate;

2.2.2 Resuspend the strain in 80μL DNA extraction solution;

2.2.3 Vortex to mix completely then incubates the mixture at 95 for 10 min;

2.2.4 Centrifuge for 2 min at 10,000 r/min;

2.2.5 The supernatant is the DNA template. Transfer the supernatant into a fresh 1.5mL tube which can be stored at -20 for long term.


2.3 The presumptive positive colony


2.3.1 Pluck the presumptive positive colony and transfer to 80μL of DNA extraction solution;

2.3.2 Vortex to mix completely then incubates the mixture at 95 for 10 min;

2.3.3 Centrifuge for 2 min at 10,000 r/min;

2.3.4 Repeat 2.2.5.


2.4 The dairy pre-enrichment
 

2.4.1 Shake the pre-enrichment culture gently and allow the mixture to stand for precipitating;

2.4.2 Insert the pipette tip to the beneath 2-3 cm of surface overlay and transfer200μL culture into a fresh 1.5mL tube;

2.4.3 Centrifuge for 2 min at 14,000 r/min and remove the supernatant completely but not convolute the precipitate;

2.4.4 If the tube wall is full of adhering drops, shot spin for 10-15s. If not, skip to the next step;

2.4.5 Resuspend the strain in 80μL DNA extraction solution;

2.4.6 Vortex to mix completely then incubates the mixture at 95 for 10 min;

 

2.4.7 Centrifuge for 2 min at 14,000 r/min. The mixture will be separated into three layers including white sediment, transparent middle layer and white floating substance. Transfer the transparent middle layer to a fresh tube carefully as to not inject floating substance and the sediment (the transparent middle layer is the DNA template).


3 Packing&shipping

We are contract member of  DHL / UPS / Fedex /TNT and provide door to door delivery services

4 Service & Technical Support


8:00-17:30 (UTC+8)  free telephone technical help service 

Free technical skills trainning in Beijing Base(3 workdays, charge 400USD/day for extra days training)

Free exchange if the products are invalid

Customers' country training is provided, charge 500USD/Day/8hours


5 FAQ

 

Q1. Can I get free samples ?

 

A1: Yes, a free half kit (48tests) is provided as the demo kit, but the freight should be paid by your side.

 

Q2. Can I get the full catalog from your side ?

 

A2: Yes, pls send your request to the official email 

 

Q3. Is Kwinbon a big company or a small company ?

 

A3: Kwinbon established in 2002 and now is China's Largest and strongest company in food safety products field. Kwinbon has more than 500 empolyees and 4 branch companies.

Kwinbon has over 10,000 square meters R&D laboratories, SPF animal house, and GMP factory. The R&D lab is equipped with instrument like GC, HPLC, LC-MS/MS. We also have 17 local sales offices 

 

Q4. Is Kwinbon professional ?

 

A4:  Kwinbon has established close collaborations with China CDC, Association of Food Science and Technology, Chinese Academy of Agriculture Science, National Institute of Food Quality Supervision and Inspection, China Agricultural University. Among the staffs, there are 9 doctors, 46 masters and 12 senior engineers, etc.

 

Q5. Is Kwinbon certificated ?

 

A5: Kwinbon passed " ISO9001:2008 " and " ISO13485:2003"

Kwinbon also won the "2011 Fobes China Best SEMs" certificates,
Kwinbon won about 100 patents in food safety detection field.

 

Q6: How is the QC ?

 

A6: Kwinbon has about 20 professional technicals in QC dept. and we will do 3 times STRICT QC for every batch of products

 

Q7. Payment terms ?

 

A7:  100% T/T in advance, L/C, Western Union 

 

Q8. Lead time ?

 

A8: For the order less than 10boxes: within 3 workdays after the fully payment

       For the order more than 10boxes: within 5 workdays after the fully payment

       For the contract order more than 200boxes: Date indicated by the customer

 

Q9. Delivery 

 

A9: Your own express carieer agent is accpet, we also provide DHL/TNT/UPS/Fedex delivery service, you can refer to your local express company for the delivery period

 

Q10. Customs clearance

 

A10: We will send you the customs clearance documents needed in advance, and help you to do the customs clearance all the time

 

Q11. OEM

 

A11. Only for the order more than 100 boxes 

 

Q12. Can I be your distributor ?

 

A12: Yes, we have many distributors all over world, some are exclusive, some are not, so please contact us for details

 

 

 

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